Glycosylation of human recombinant gonadotrophins: characterization and batch-to-batch consistency

Fed-batch Cultivation of Recombinant Escherichia coli Producing Human Interferon-γ Under Controlled Specific Growth Rate

Evaluation of Heat Induction Strategy for Recombinant Human Growth Hormone Expression in Fed-Batch Fermentation

Production of recombinant human annexin V by fed-batch cultivation

BACKGROUND Annexin V, a 35.8 kDa intracellular protein, is a Ca⁺²-dependent phospholipid binding protein with high affinity to phosphatidylserine (PS), which is a well-known hallmark of apoptosis. Annexin V is a sensitive probe for PS exposure upon the cell membrane, and used for detection of apoptotic cells both in vivo and in vitro. Large-scale production of recombinant human annexin V is wor...

Single-Batch Production of Recombinant Human Polyclonal Antibodies

We have previously described the development and implementation of a strategy for production of recombinant polyclonal antibodies (rpAb) in single batches employing CHO cells generated by site-specific integration, the Sympress I technology. The Sympress I technology is implemented at industrial scale, supporting a phase II clinical development program. Production of recombinant proteins by sit...

Batch Roundness Characterization and Evaluation

A new concept referred to as the batch roundness is presented in this paper. This concept is derived to illustrate the worst possible form error for a batch of circular features machined under the same conditions. It is to be used as a statistical quality measure for such a batch of circular features and evaluated by analyzing their systematic and random form error components. The definition of...